Journal: American Journal of Physiology - Endocrinology and Metabolism
Article Title: Aerobic capacity and hepatic mitochondrial lipid oxidation alters susceptibility for chronic high-fat diet-induced hepatic steatosis
doi: 10.1152/ajpendo.00178.2016
Figure Lengend Snippet: Chronic HFD differentially changes mitochondrial protein acetylation and succinylation states in HCR and LCR rats. Total protein acetylation and succinylation levels and mitochondrial protein content of enzymes that putatively control these levels were measured by Western blot. A: sirtuin (Sirt)3. B: protein acetylation. C: Sirt5. D: protein succinylation. E: representative blots. All values are means ± SE; n = 8–16. §P < 0.05, interaction; +P < 0.05, main effect of strain; &P < 0.05, main effect of diet (45% vs. 60% HFD); †P < 0.05, main effect of HFD vs. LFD; ††P < 0.05, difference in HFD vs. LFD within strain.
Article Snippet: SOD2, sirtuin (Sirt)3, Sirt5, and acetylated lysine antibodies were purchased from Cell Signaling Technologies (Danvers, MA).
Techniques: Control, Western Blot